MEDIR: METABOLIC DISORDERS

Paula Macedo

M. Paula Macedo

Principal Investigator

CV

Location:

CEDOC
Campus Sant'Ana
Pólo de Investigação, NMS, UNL
Rua Câmara Pestana, nº 6
1150-082 Lisboa, Portugal

Phone:
(+351) 218 803 017
(+351) 218 803 000 (ext. 20343)
Fax: (+351) 218 851 920
E-mail: paula.macedo(at)nms.unl.pt

Main interests

Prediabetes and Diabetes – Etiological processes and associated pathologies.

Research Areas

-Prandial State Metabolic Control
- Carbohydrate and Lipid Metabolism
-Autonomic Nervous System
-Mechanisms of Progression to Prediabetes and Diabetes
-Aging
-Obesity and Inflammation
-Diet-induced Dysmetabolism
-Protein Expression and Activity
-Insulin Resistance and Hypertension
-Myocardial Dysfunction
-Cardiorenal Risk
-Evaluation of Prediabetes in specific Populations

metabolic-areas

metabolic-projects

Projects

Ongoing Projects:
Fundação Ciência e Tecnologia PTDC/BIM-MET/0486/2012 - €172.888 - Principal investigator
CD26/DPP4 in Fatty Liver Disease progression: inflammation impinges on dysmetabolism.

Fundação Ciência e Tecnologia PTDC/DTP-EPI/0207/2012 - €199.288 - Principal investigator
Insulin Clearance (Dys)Regulation: clarifying the mist.

Fundação Ciência e Tecnologia EXCL/DTP-PIC/0069/2012 - €350000 - Team member
Anticipating diabetes and diabetic wounding for siblings of Type 2 diabetics.

Fundação Ciência e Tecnologia EXPL/DTP-PIC/0244/2012 - €45.647 - Team member
Glycated albumin as an avenue of progression to Diabetes: From new molecular mechanisms to clinical relevance on the Portuguese population.

Previous Projects:
“Meal-induced insulin sensitization (MIS) - from basics to clinical studies”
Fundação para a Ciência e Tecnologia
2007-2012

“Condição do Sistema Nervoso Parassimpático e Intolerância à Glucose”
Sociedade Portuguesa de Diabetologia/Bayer
2010-2011

“Os níveis de retinol-binding protein 4 (RBP4) influenciam a sensibilidade à insulina no estado pós-prandial”
Sociedade Portuguesa de Diabetologia/Novo Nordisk e CEDOC
2010-2011

“Estudo da Prevalência da Diabetes em Portugal”
Sociedade Portuguesa de Diabetologia, Associação Protectora dos Diabéticos de Portugal, Instituto de Higiene e Medicina Social – FM-UC, Direcção-Geral de Saúde, Programa Nacional de Prevenção e Controlo da Diabetes
2008-2010

"Sweet"
Executive Agency for Health and consumers (EAHC)
2008-2011

"MGSD"
2011-2013

Publications
  • Delgado, T.C., Martins, F.O., Carvalho, F., Goncalves, A., Scott, D.K., O'Doherty, R., Paula Macedo, M., and Jones, J.G. (2013). H-2 enrichment distribution of hepatic glycogen from (H2O)-H-2 reveals the contribution of dietary fructose to glycogen synthesis. American Journal of Physiology-Endocrinology and Metabolism 304, E384-E391.
  • Lima, I.S., Huang, H., Lee, S.H., Jarak, I., Jones, J.G., Macedo, M.P., and Kim, Y.B. (2013). Deletion of hepatic ROCK1 prevents steatosis by reducing lipid synthesis and activating autophagic flux in diet-induced obese mice. Diabetologia 56, S313-S313.
  • Martins, F.O., Rito, J., Jarak, I., Viegas, I., Pardal, M.A., Paula Macedo, M., and Jones, J.G. (2013). Disposition of U-H-2(7) glucose into hepatic glycogen in rat and in seabass. Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology 166, 316-322.
  • Ribeiro, R.T., Macedo, M.P., Gardete-Correia, L., Duarte, R., Boavida, J.M., Correia, I., Peerally, Z., Martins, F.O., and Raposo, J.F. (2013). Fatty liver disease accompanies loss of glycaemic control on the 5 years follow-up of a previously non-diabetic population. Diabetologia 56, S178-S179.
  • Trico, D., Tulipani, A., Baldi, S., Trifiro, S., Frascerra, S., Macedo, M.P., Mari, A., Ferrannini, E., and Natali, A. (2013). Effects of a small protein and lipid preload on oral glucose tolerance in subjects with normal and impaired glucose tolerance. Diabetologia 56, S101-S101.
  • Afonso, R.A., Fernandes, A.B., Santos, C., Ligeiro, D., Ribeiro, R.T., Lima, I.S., Patarrao, R.S., Videira, P.A., Caldeira, J., and Macedo, M.P. (2012). Postprandial Insulin Resistance in Zucker Diabetic Fatty Rats is Associated with Parasympathetic-Nitric Oxide Axis Deficiencies. Journal of Neuroendocrinology 24, 1346-1355.
  • Fernandes, A.B., Guarino, M.P., and Paula Macedo, M. (2012). Understanding the in-vivo relevance of S-nitrosothiols in insulin action. Canadian Journal of Physiology and Pharmacology 90, 887-894.
  • Martins, F.O., Jones, J.G., Ribeiro, R., Mari, A., Natali, A., and Macedo, M.P. (2012). In vivo insulin clearance regulation: a role for nitric oxide. Diabetologia 55, S251-S252.
  • Patarrao, R.S., Lautt, W.W., Afonso, R.A., Ribeiro, R.T., Fernandes, A.B., Boavida, J.M., and Paula Macedo, M. (2012). Postprandial but not fasting insulin resistance is an early identifier of dysmetabolism in overweight subjects. Canadian Journal of Physiology and Pharmacology 90, 923-931.
  • Fernandes, A.B., Lima, I.S., and Macedo, M.P. (2011). S-nitrosothiols as potential pharmacological targets on the treatment of insulin resistance. Diabetologia 54, S252-S252.
  • Fernandes, A.B., Patarrao, R.S., Videira, P.A., and Macedo, M.P. (2011). Understanding Postprandial Glucose Clearance by Peripheral Organs: The Role of the Hepatic Parasympathetic System. Journal of Neuroendocrinology 23, 1288-1295.
  • Lautt, W.W., Schafer, J., Macedo, M.P., and Legare, D.J. (2011). Bethanechol and N-acetylcysteine mimic feeding signals and reverse insulin resistance in fasted and sucrose-induced diabetic rats. Canadian Journal of Physiology and Pharmacology 89, 135-142.
  • Lima, I.S., Fernandes, A.B., Jones, J.G., Manadas, B., Mendes, V., and Macedo, M.P. (2011). Altered glucose excursions during a meal tolerance test in an impaired hepatic vagal model are mainly due to peripheral insulin resistance. Diabetologia 54, S55-S56.
  • Patarrao, R.S., Fernandes, A.B., Lima, I.S., and Macedo, M.P. (2011). Impairment of insulin clearance rather than beta cell function in the progression to type 2 diabetes. Diabetologia 54, S237-S238.
  • Afonso, R.A., Lautt, W.W., Schafer, J., Legare, D.J., Oliveira, A.G., and Paula Macedo, M. (2010). High-fat diet results in postprandial insulin resistance that involves parasympathetic dysfunction. British Journal of Nutrition 104, 1450-1459.
  • Fernandes, A.B., Videira, P.A., Bonito, N., Patarrao, R.S., Afonso, R.A., and Macedo, M.P. (2009). Postprandial glucose disposal is subject to hepatic parasympathetic control. Diabetologia 52, S222-S222.
  • Macedo, M.P., Bonito, N., and Fernandes, A.B. (2009). S-nitrosothiols as insulin resistance reversers. Diabetologia 52, S223-S223.
  • Lautt, W.W., Ming, Z., Macedo, M.P., and Legare, D.J. (2008). HISS-dependent insulin resistance (HDIR) in aged rats is associated with adiposity, progresses to syndrome X, and is attenuated by a unique antioxidant cocktail. Experimental Gerontology 43, 790-800.
  • Patarrao, R.S., Lautt, W.W., Afonso, R.A., Ribeiro, R.T., Guarino, M.P., Fernandes, A.B., Boavida, J.M., and Macedo, M.P. (2008). Meal-induced insulin sensitization and its parasympathetic regulation in humans. Canadian Journal of Physiology and Pharmacology 86, 880-888.
  • Afonso, R.A., Lautt, W.W., Schafer, J., Legare, D.J., and Macedo, M.P. (2007). High-fat diet decreases meal-induced insulin sensitivity by impairing hepatic parasympathetic pathway, which is responsible for insulin resistance. Diabetologia 50, S265-S265.
  • Afonso, R.A., Ribeiro, R.T., Fernandes, A.B., Patarrao, R.S., and Macedo, M.P. (2007). Hepatic-dependent and -independent insulin actions are impaired in the obese Zucker rat model. Obesity 15, 314-321.
  • Fernandes, A.B., Videira, P., Guarino, M.P., Ribeiro, R., Afonso, R.A., and Macedo, M.P. (2007). Hepatic nitric oxide synthase activity and expression related to insulin resistance in an ageing obesity animal model. Diabetologia 50, S304-S304.
  • Ribeiro, R.T., Afonso, R.A., and Macedo, M.P. (2007). Hepatic parasympathetic role in insulin resistance on an animal model of hypertension. Metabolism-Clinical and Experimental 56, 227-233.
  • Ribeiro, R.T., Fernandes, A.B., Afonso, R.A., Videira, P.A., Correia, M., and Macedo, M.P. (2007). Hepatic iNOS and ST6Gall inflammatory markers are increased in obese diabetic but not in nondiabetic obese rat models. Diabetologia 50, S303-S304.
  • Schafer, J., Macedo, M.P., Legare, D.J., and Lautt, W.W. (2007). Reversing prediabetic insulin resistance by a synergy of drugs mimicking a feeding signal. Diabetes 56, A550-A550.
  • Afonso, R.A., Patarrao, R.S., Macedo, M.P., and Carmo, M.M. (2006). Carvedilol action is dependent on endogenous production of nitric oxide. American Journal of Hypertension 19, 419-425.
  • Guarino, M.P., and Macedo, M.P. (2006). Co-administration of glutathione and nitric oxide enhances insulin sensitivity in Wistar rats. British Journal of Pharmacology 147, 959-965.
  • Macedo, M.P., Ribeiro, R.T., Afonso, R.A., and Fernandes, A. (2006). Impairment of Parasympathetic-dependent and independent insulin action in Zucker Diabetic Fatty rats. Acta Pharmacologica Sinica 27, 307-308.
  • Santos, C., Afonso, R.A., Guarino, M.P., Patarrao, R.S., Fernandes, A., Noronha, J.P., Macedo, M.P., and Calcleira, J. (2006). In vitro nitrosation of insulin A- and B-chains. European Journal of Mass Spectrometry 12, 331-338.
  • Ribeiro, R., Lautt, W., Legare, D., and Macedo, M. (2005). Insulin resistance induced by sucrose feeding in rats is due to an impairment of the hepatic parasympathetic nerves. Diabetologia 48, 976-983.
  • Afonso, R.A., Patarra, R.S., Macedo, M.P., Rendas, A., and Carmo, M.M. (2004). Is the pharmacological action of carvedilol nitric oxide dependent? Fundamental & Clinical Pharmacology 18, 61-61.
  • Guarino, M.P., Correia, N.C., Lautt, W.W., and Macedo, M.P. (2004). Insulin sensitivity is mediated by the activation of the ACh/NO/cGMP pathway in rat liver. American Journal of Physiology-Gastrointestinal and Liver Physiology 287, G527-G532.
  • Guarino, M.P., and Macedo, M.P. (2004). Glutathione monoethyl ester induces a dose dependent increase of insulin sensitivity in wistar rats. Fundamental & Clinical Pharmacology 18, 66-66.
  • Lautt, W.W., Macedo, M.P., Sadri, P., Legare, D.J., Reid, M.A.G., and Guarino, M.P. (2004). Pharmaceutical reversal of insulin resistance. Proceedings of the Western Pharmacology Society 47, 30-32.
  • Guarino, M.P., Afonso, R.A., Raimundo, N., Raposo, J.F., and Macedo, M.P. (2003). Hepatic glutathione and nitric oxide are critical for hepatic insulin-sensitizing substance action. American Journal of Physiology-Gastrointestinal and Liver Physiology 284, G588-G594.
  • Guarino, M.P., Correia, N.C., Raposo, J., and Macedo, M.P. (2001). Nitric oxide synthase inhibition decreases output of hepatic insulin sensitizing substance (HISS), which is reversed by SIN-1 but not by nitroprusside. In Proceedings of the Western Pharmacology Society, R.J. Huxtable, ed., pp. 25-26.
  • Patarrao, R.S., Macedo, M.P., Raposo, J.F., and Carmo, M.M. (2001). Hepatic metabolic effects of norepinephrine are potentiated by nitric oxide. In Proceedings of the Western Pharmacology Society, R.J. Huxtable, ed., pp. 23-24.
  • Ribeiro, R.T., Duarte-Ramos, F., and Macedo, M.P. (2001). The action of hepatic insulin sensitizing substance is decreased in rats on a high-sucrose diet. In Proceedings of the Western Pharmacology Society, R.J. Huxtable, ed., pp. 31-32.
  • Ribeiro, R.T., Duarte-Ramos, F., and Macedo, M.P. (2001). Effect of the hepatic insulin sensitizing substance in the spontaneously hypertensive rat. In Proceedings of the Western Pharmacology Society, R.J. Huxtable, ed., pp. 27-28.
  • Ribeiro, R.T., Duarte-Ramos, F., and Macedo, M.P. (2001). The fatty Zucker rat fa/fa shows a dysfunction of the HISS-dependent and -independent components of insulin action. In Proceedings of the Western Pharmacology Society, R.J. Huxtable, ed., pp. 29-30.
  • Lautt, W.W., and Macedo, M.P. (1997). Hepatic circulation and toxicology. Drug Metabolism Reviews 29, 369-395.
  • Lautt, W.W., Macedo, M.P., Sadri, P., Takayama, S., Ramos, F.D., and Legare, D.J. (2001). Hepatic parasympathetic (HISS) control of insulin sensitivity determined by feeding and fasting. American Journal of Physiology-Gastrointestinal and Liver Physiology 281, G29-G36.
  • Sebastiao, A.M., Macedo, M.P., and Ribeiro, J.A. (2000). Tonic activation of A(2A) adenosine receptors unmasks, and of A(1) receptors prevents, a facilitatory action of calcitonin gene-related peptide in the rat hippocampus. British Journal of Pharmacology 129, 374-380.
  • Lautt, W.W., Wang, X.S., Sadri, P., Legare, D.J., and Macedo, M.P. (1998). Rapid insulin sensitivity test (RIST). Canadian Journal of Physiology and Pharmacology 76, 1080-1086.
  • Macedo, M.P., and Lautt, W.W. (1998). Shear-induced modulation of vasoconstriction in the hepatic artery and portal vein by nitric oxide. American Journal of Physiology-Gastrointestinal and Liver Physiology 274, G253-G260.
  • Macedo, M.P., and Lautt, W.W. (1997). Potentiation to vasodilators by nitric oxide synthase blockade in superior mesenteric but not hepatic artery. American Journal of Physiology-Gastrointestinal and Liver Physiology 272, G507-G514.
  • Macedo, M.P., and Lautt, W.W. (1996). Autoregulatory capacity in the superior mesenteric artery is attenuated by nitric oxide. American Journal of Physiology-Gastrointestinal and Liver Physiology 271, G400-G404.
  • Macedo, M.P., and Lautt, W.W. (1996). Shear-induced modulation by nitric oxide of sympathetic nerves in the superior mesenteric artery. Canadian Journal of Physiology and Pharmacology 74, 692-700.
  • Macedo, M.P., and Lautt, W.W. (1995). NITRIC OXIDE SYNTHASE ANTAGONISM POTENTIATES PRESSURE-FLOW AUTOREGULATION IN THE SUPERIOR MESENTERIC ARTERY. Western Pharmacology Society, Proceedings of the Thirty-Eighth Annual Meeting 38, 33-34.
  • Macedo, M.P., and Lautt, W.W. (1994). NITRIC-OXIDE SUPPRESSION OF NOREPINEPHRINE RELEASE FROM NERVES IN THE SUPERIOR MESENTERIC-ARTERY. Proceedings of the Western Pharmacology Society, Vol 37, 1994 37, 103-104.

Collaborations

- Faculty of Medicine, University of Manitoba - Canada

- Institute of Biomedical Engineering, University of Padua -Italy

- CNC, University of Coimbra - Portugal

- University of Pittsburgh - USA

- BIDMC – Endocrinology, Harvard University - USA

- FCT/REQUIMTE - Portugal

Team photos

fotografia grupo medir

metabolic-team